PAMS Seminar: "Understanding Molecular Machines using Protein and DNA Origami Nanoarrays" by Dr. Rizal Hariadi

Dr. Rizal Hariadi
Department of Physics and the Biodesign Institute
Arizona State University

Abstract:
The global dynamics of complex biomolecular systems arise, in most part, from the organization and weak interactions of molecular machines. Programmable DNA origami, in combination with conjugation strategies, offers a unique opportunity to organize biomolecules (such as proteins) for applications in fundamental biological physics studies and diagnostics. In this talk, I will present our latest research demonstrating the exquisite positional control of DNA origami technology across multiple length scales. In the first part of my talk, I will show two DNA-nanostructure-guided protein arrays for dissecting collective behaviors of molecular machines consisting of 100-nm-sized rectangular DNA origami nanostructures and tens-of-micron-long DNA nanotubes, patterned with myosin motors. These studies uncover elegant biophysical principles for designing force sensors and molecular transporters on complex landscapes. In the second half of the talk, I will present some recent results in integrating bottom-up DNA origami nanostructures with top-down lithography for cm-scale protein nanoarrays. Finally, I will conclude with a discussion on the transformative potential and future directions of protein and DNA nanoarrays, including high-throughput single-molecule biophysics measurements.

This seminar will be held exclusively on Zoom (955 5209 1021). Please visit the Physics Seminars page for a link.

Free